Supporting Information & Loose Ends Project Files
Supporting Information
This section of the website provides supporting information for publications.
Supporting Information
The new OSX11.xx 'MicroChipAnalysis' application includes RNAseq information when available. This summary (which is also part of the help file in the application) describes some analysis and integration of this information into the application.
Supporting Information
This document contains an expanded methods for a report published in Clinical and Experimental Allergy "Efficacy of the 4 Subclasses of IgG for Interacting With CD32b/a on Human Basophils" online (ahead of print). Due to space limitations in the research letter, some of the data is presented in the 'Loose Ends Project' document below summarizing the 10 years of evidence describing basophil CD32 function. See the section on subclass efficacy. Note: this extended synopsis (PDF-09) will be added to this website within a few weeks of the CEA publication.
Loose Ends Project (LEP)
This section of the website includes documents related to the 'Loose Ends Project'. A brief background description of this project is linked to the PDF button.
LEP-Desensitization
All receptor-mediated activation requires signaling events that reverse the activating steps so that the cell returns to its resting or new phenotypic state. This self-termination or desensitization has been a domain of study in our laboratory to understand IgE-mediated activation. Several decades of study is summarized in this document. Like all 'Loose Ends Project' reports, this synopsis includes many unpublished experiments (with full experimental details).
LEP-Desensitization
Examining the role of protein kinase C (PKC) isozymes during basophil and mast cell activation was one of the efforts to understand desensitization. This document summarizes our studies of PKC and phorbol ester-mediated activation. Included are recent unpublished studies that discuss how the PMA-curve shift behavior might be used to explore whether basophils experience IgE-mediated stimulation in vivo using an ex vivo assay.
LEP-Desensitization
Monoclonal vs. polyclonal anti-IgE antibodies for stimulation: differential characteristics
LEP-Regulation
For a couple of decades this laboratory has explored the role and expression of SYK in the human basophil and mast cell IgE-mediated response. The most recent aspect of this effort is to examine regulation of SYK gene transcription. These are incomplete studies in that resolution of some of the issues is not yet in hand. That said, there are interesting and suggestive experiments that will not be further pursued since this lab is closed.
LEP-Regulation
Supporting studies and analysis on regulating IgE-mediated secretion from human basophils by CD32b or CD32a.